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Western blot(SDS PAGE) analysis of extracts from Ramos cell lysate.Using Bmf Rabbit mAb [CO00]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :Bcl2 modifying factor; Bmf;
UniProtID :Q96LC9
MASS(da) :20,508
MW(kDa) :24kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Bmf
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Ramos cell lysate.
Function : May play a role in apoptosis. Isoform 1 seems to be the main initiator.
Tissue specificity :Isoform 1 is mainly expressed in B-lymphoid cells. Isoform 2 and isoform 3 are mainly expressed in B-CLL and normal B-cells..
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.