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ENT2 Rabbit mAb [ybFb]Cat NO.: A65130

Western blot(SDS PAGE) analysis of extracts from K562 cell lysate.Using ENT2 Rabbit mAb [ybFb]at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :DER12; ei-type; HNP36; Nucleoside transporter; SLC29A2;

UniProtID :Q14542

MASS(da) :50,113

MW(kDa) :55-65kDa

Form :Liquid

Purification :Affinity-chromatography

Host :Rabbit

Isotype : IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF)1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human ENT2

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :K562 cell lysate.

Function : Mediates equilibrative transport of purine, pyrimidine nucleosides and the purine base hypoxanthine. Very less sensitive than SLC29A1 to inhibition by nitrobenzylthioinosine (NBMPR), dipyridamole, dilazep and draflazine..

Tissue specificity :Expressed in skeletal muscle, liver, lung, placenta, brain, heart, kidney and ovarian tissues..

Subcellular locationi :Basolateral cell membrane,Multi-pass membrane protein. Nucleus membrane,Multi-pass membrane protein.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.