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Western blot(SDS PAGE) analysis of extracts from Human fetal muscle lysate.Using MT-ND1 Rabbit mAb [Fmm5]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :MT-ND1; MTND1; NAD1; NADH1; ND1;
UniProtID :P03886
MASS(da) :35,661
MW(kDa) :38kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human MT-ND1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Human fetal muscle lysate.
Function : Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor (PubMed:1959619). Essential for the catalytic activity and assembly of complex I (PubMed:26929434, PubMed:1959619)..
Subcellular locationi :Mitochondrion inner membrane,Multi-pass membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.