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LDHB Rabbit mAb [5052]Cat NO.: A77720

Western blot(SDS PAGE) analysis of extracts from HeLa cell lysate.Using LDHB Rabbit mAb [5052]at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :HEL S 281; LDH B; LDH H; LDH-B; LDH-H; LDHB; LDHBD; LDHH; TRG5;

UniProtID :P07195

MASS(da) :36,638

MW(kDa) :37kDa

Form :Liquid

Purification :Affinity-chromatography

Host :Rabbit

Isotype : IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF)1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human LDHB

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HeLa cell lysate.

Function : Interconverts simultaneously and stereospecifically pyruvate and lactate with concomitant interconversion of NADH and NAD(+)..

Tissue specificity :Predominantly expressed in aerobic tissues such as cardiac muscle..

Subcellular locationi :Cytoplasm. Mitochondrion inner membrane,Peripheral membrane protein.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.