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Western blot(SDS PAGE) analysis of extracts from HeLa cell lysate.Using LDHB Rabbit mAb [5052]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :HEL S 281; LDH B; LDH H; LDH-B; LDH-H; LDHB; LDHBD; LDHH; TRG5;
UniProtID :P07195
MASS(da) :36,638
MW(kDa) :37kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human LDHB
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cell lysate.
Function : Interconverts simultaneously and stereospecifically pyruvate and lactate with concomitant interconversion of NADH and NAD(+)..
Tissue specificity :Predominantly expressed in aerobic tissues such as cardiac muscle..
Subcellular locationi :Cytoplasm. Mitochondrion inner membrane,Peripheral membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.