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alpha 1 Spectrin Rabbit mAb [ep83]Cat NO.: A15351

Western blot(SDS PAGE) analysis of extracts from K562 cell lysate.Using alpha 1 Spectrin Rabbit mAb [ep83]at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :EL2; Elliptocytosis2; erythrocyte; Erythroid alpha spectrin; HPP; HS3; SPH3; SPTA; SPTA1;

UniProtID :P02549

MASS(da) :280,014

MW(kDa) :280kDa

Form :Liquid

Purification :Affinity-chromatography

Host :Rabbit

Isotype : IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫荧光(ICC/IF)1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human alpha 1 Spectrin

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :K562 cell lysate.

Function : Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane.

Subcellular locationi :Cytoplasm, cytoskeleton. Cytoplasm, cell cortex.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.