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Western blot(SDS PAGE) analysis of extracts from K562 cell lysate.Using alpha 1 Spectrin Rabbit mAb [ep83]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :EL2; Elliptocytosis2; erythrocyte; Erythroid alpha spectrin; HPP; HS3; SPH3; SPTA; SPTA1;
UniProtID :P02549
MASS(da) :280,014
MW(kDa) :280kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human alpha 1 Spectrin
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :K562 cell lysate.
Function : Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane.
Subcellular locationi :Cytoplasm, cytoskeleton. Cytoplasm, cell cortex.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.