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Western blot(SDS PAGE) analysis of extracts from HeLa cell lysate.Using BACH1/BRIP1 Rabbit mAb [kv3b]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :BACH 1; BRIP 1; BRIP1; FANCJ;
UniProtID :Q9BX63
MASS(da) :140,867
MW(kDa) :105kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human BACH1/BRIP1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cell lysate.
Function : DNA-dependent ATPase and 5' to 3' DNA helicase required for the maintenance of chromosomal stability. Acts late in the Fanconi anemia pathway, after FANCD2 ubiquitination. Involved in the repair of DNA double-strand breaks by homologous recombination in a manner that depends on its association with BRCA1..
Tissue specificity :Ubiquitously expressed, with highest levels in testis..
Subcellular locationi :Nucleus. Cytoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.