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Western blot(SDS PAGE) analysis of extracts from Jurkat cell lysate.Using THRA Rabbit mAb [MT44]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :AR7; CHNG6; EAR7; ERB-T-1; ERBA; ERBA1; NR1A1; THRA; THRA1; THRA2;
UniProtID :P10827
MASS(da) :54,816
MW(kDa) :55kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human THRA
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Jurkat cell lysate.
Function : [Isoform Alpha-1]: Nuclear hormone receptor that can act as a repressor or activator of transcription. High affinity receptor for thyroid hormones, including triiodothyronine and thyroxine.., [Isoform Alpha-2]: Does not bind thyroid hormone and functions as a weak dominant negative inhibitor of thyroid hormone action..
Subcellular locationi :Nucleus.,[Isoform Alpha-2]: Cytoplasm. Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.