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Western blot(SDS PAGE) analysis of extracts from Raji cell lysate.Using NCF1 Rabbit mAb [26Jt]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :NCF1; NCF1A; NOXO2; SH3PXD1A; p47phox;
UniProtID :P14598
MASS(da) :44,682
MW(kDa) :45kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human NCF1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Raji cell lysate.
Function : NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production)..
Tissue specificity :Detected in peripheral blood monocytes and neutrophils (at protein level)..
Subcellular locationi :Cytoplasm, cytosol. Membrane,Peripheral membrane protein,Cytoplasmic side.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.