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Western blot(SDS PAGE) analysis of extracts from 293T cell lysate.Using Engrailed 1 Rabbit mAb [E4t3]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :EN1; Engrailed 1; Engrailed; Hu-En-1; Mo-en.1;
UniProtID :Q05925
MASS(da) :40,115
MW(kDa) :55kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Engrailed 1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :293T cell lysate.
Function : Required for proper formation of the apical ectodermal ridge and correct dorsal-ventral patterning in the limb..
Subcellular locationi :Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.