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Engrailed 1 Rabbit mAb [E4t3]Cat NO.: A73568

Western blot(SDS PAGE) analysis of extracts from 293T cell lysate.Using Engrailed 1 Rabbit mAb [E4t3]at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :EN1; Engrailed 1; Engrailed; Hu-En-1; Mo-en.1;

UniProtID :Q05925

MASS(da) :40,115

MW(kDa) :55kDa

Form :Liquid

Purification :Affinity-chromatography

Host :Rabbit

Isotype : IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Engrailed 1

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :293T cell lysate.

Function : Required for proper formation of the apical ectodermal ridge and correct dorsal-ventral patterning in the limb..

Subcellular locationi :Nucleus.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.