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Western blot(SDS PAGE) analysis of extracts from HeLa cell lysate.Using UNG Rabbit mAb [38F5]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :UNG; DGU; HIGM4; HIGM5; Uracil-DNA glycosylase; UNG1; UNG2; UDG; UNG15;
UniProtID :P13051
MASS(da) :34,645
MW(kDa) :32kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human UNG
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cell lysate.
Function : Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine.
Tissue specificity :Isoform 1 is widely expressed with the highest expression in skeletal muscle, heart and testicles. Isoform 2 has the highest expression levels in tissues containing proliferating cells.
Subcellular locationi :[Isoform 1]: Mitochondrion.,[Isoform 2]: Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.