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Western blot(SDS PAGE) analysis of extracts from U87-MG cell lysate.Using TFPI Rabbit mAb [bBu2]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :EPI; LACI; TFI; TFPI1;
UniProtID :P10646
MASS(da) :35,015
MW(kDa) :35-45kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human TFPI
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :U87-MG cell lysate.
Function : Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma.
Tissue specificity :Mostly in endothelial cells.
Subcellular locationi :[Isoform Alpha]: Secreted.,[Isoform Beta]: Microsome membrane,Lipid-anchor, GPI-anchor.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.