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Western blot(SDS PAGE) analysis of extracts from Mouse brain.Using VGLUT2 Rabbit mAb [62FS] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :SLC17A6,DNPI,VGLUT2,VGLU2_HUMAN,Vesicular glutamate transporter 2 -dependent inorganic phosphate cotransporter)
UniProtID :Q9P2U8
MASS(da) :64,392
MW(kDa) :65-70 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human VGLUT2
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Mouse brain
Function : Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells (PubMed:11698620). May also mediate the transport of inorganic phosphate (PubMed:10820226). Involved in the regulation of retinal hyaloid vessel regression during postnatal development (By similarity)..
Tissue specificity :Predominantly expressed in adult brain (PubMed:10820226). Expressed in amygdala, caudate nucleus, cerebral cortex, frontal lobe, hippocampus, medulla, occipital lobe, putamen, spinal cord, substantia nigra, subthalamic nucleus, temporal lobe and thalamus (PubMed:10820226)..
Subcellular locationi :Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane,Multi-pass membrane protein. Cell junction, synapse, synaptosome.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.