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Western blot(SDS PAGE) analysis of extracts from A375 cell lysate.Using ECM1 Rabbit mAb [4T42]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :Ecm1; URBWD;
UniProtID :Q16610
MASS(da) :60,674
MW(kDa) :70kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human ECM1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :A375 cell lysate.
Function : Involved in endochondral bone formation as negative regulator of bone mineralization. Stimulates the proliferation of endothelial cells and promotes angiogenesis. Inhibits MMP9 proteolytic activity..
Tissue specificity :Expressed in breast cancer tissues. Little or no expression observed in normal breast tissues. Expressed in skin,wide expression is observed throughout the dermis with minimal expression in the epidermis..
Subcellular locationi :Secreted, extracellular space, extracellular matrix.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.