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Western blot(SDS PAGE) analysis of extracts from human heart lysate.Using Monoacylglycerol Lipase Rabbit mAb [yX0F]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :HUK5; Lysophospholipase homolog; Lysophospholipase like; MAGL; MGL; MGLL;Monoacylglycerol lipase;
UniProtID :Q99685
MASS(da) :33,261
MW(kDa) :33kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Monoacylglycerol Lipase
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :human heart lysate.
Function : Converts monoacylglycerides to free fatty acids and glycerol (PubMed:19029917, PubMed:20079333, PubMed:21049984, PubMed:22969151, PubMed:24368842). Hydrolyzes the endocannabinoid 2-arachidonoylglycerol, and thereby contributes to the regulation of endocannabinoid signaling, nociperception and perception of pain (PubMed:19029917, PubMed:20079333, PubMed:21049984, PubMed:22969151, PubMed:24368842). Regulates the levels of fatty acids that serve as signaling molecules and promote cancer cell migration, invasion and tumor growth (PubMed:20079333)..
Tissue specificity :Detected in adipose tissue, lung, liver, kidney, brain and heart..
Subcellular locationi :Cytoplasm, cytosol. Membrane,Peripheral membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.