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Western blot(SDS PAGE) analysis of extracts from K562 cell lysate.Using p66 alpha Rabbit mAb [NGUs]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :GATAD2A; Hp66alpha; P66 alpha; p66alpha;
UniProtID :Q86YP4
MASS(da) :68,063
MW(kDa) :65,68kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human Mouse
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human p66 alpha
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :K562 cell lysate.
Function : Transcriptional repressor. Enhances MBD2-mediated repression. Efficient repression requires the presence of GATAD2B..
Tissue specificity :Ubiquitous, both in fetal and adult tissues..
Subcellular locationi :Nucleus speckle.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.