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Western blot(SDS PAGE) analysis of extracts from MOLT-4 cells.Using WTAP Rabbit mAb [HLF2] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :WTAP,KIAA0105,FL2D_HUMAN,Pre-mRNA-splicing regulator WTAP D homolog) D)
UniProtID :Q15007
MASS(da) :44,244
MW(kDa) :55 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human WTAP
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :MOLT-4 cells
Function : Associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of RNAs, a modification that plays a role in the efficiency of mRNA splicing and RNA processing (PubMed:29507755). Required for accumulation of METTL3 and METTL14 to nuclear speckle (PubMed:24316715, PubMed:24407421, PubMed:24981863). Acts as a mRNA splicing regulator (PubMed:12444081). Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability (PubMed:17088532). Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes (PubMed:17095724)..
Tissue specificity :Ubiquitously expressed..
Subcellular locationi :Nucleus speckle. Nucleus, nucleoplasm. Cytoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.