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Western blot(SDS PAGE) analysis of extracts from A549 cell lysate.Using HDGF Rabbit mAb [2a5N]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :HDGF; HMG1L2;
UniProtID :P51858
MASS(da) :26,788
MW(kDa) :37kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human Mouse
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human HDGF
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :A549 cell lysate.
Function : [Isoform 1]: Acts as a transcriptional repressor (PubMed:17974029). Has mitogenic activity for fibroblasts (PubMed:11751870, PubMed:26845719). Heparin-binding protein (PubMed:15491618).., [Isoform 2]: Does not have mitogenic activity for fibroblasts (PubMed:26845719). Does not bind heparin (PubMed:26845719).., [Isoform 3]: Has mitogenic activity for fibroblasts (PubMed:26845719). Heparin-binding protein (PubMed:26845719)..
Tissue specificity :Ubiquitous..
Subcellular locationi :[Isoform 1]: Nucleus. Cytoplasm. Secreted, extracellular exosome.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.