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Western blot(SDS PAGE) analysis of extracts from NK-92 treated as indicated with TPA (80 nM 5 hr) Ionomycin Calcium Salt(3 μM 5 hr) and Brefeldin A(300 ng/mL last 4 hr of stimulation).Using XCL1/XCL2 Rabbit mAb [29EC] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :XCL1,LTN,SCYC1,XCL1_HUMAN,Lymphotactin
UniProtID :P47992
MASS(da) :12,517
MW(kDa) :12 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human XCL1/XCL2
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :NK-92 treated as indicated with TPA (80 nM 5 hr) Ionomycin Calcium Salt(3 μM 5 hr) and Brefeldin A(300 ng/mL last 4 hr of stimulation)
Function : Chemotactic activity for lymphocytes but not for monocytes or neutrophils. In thymus, mediates medullary accumulation of thymic dendritic cells and contributes to regulatoy T cell development, playing a role in self-tolerance establishment..
Tissue specificity :Highest level in spleen, lower in peripheral leukocytes and very low levels in lung, colon and small intestine.
Subcellular locationi :Secreted.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.