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MYL12B Rabbit mAb [4s5I]Cat NO.: A86740

Western blot(SDS PAGE) analysis of extracts from Jurkat cell lysate.Using MYL12B Rabbit mAb [4s5I]at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :MLC 2A; MLC20; MRLC2; MYL12B; MYLC2B;

UniProtID :O14950

MASS(da) :19,779

MW(kDa) :18kDa

Form :Liquid

Purification :Affinity-chromatography

Host :Rabbit

Isotype : IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF)1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human MYL12B

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :Jurkat cell lysate.

Function : Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Phosphorylation triggers actin polymerization in vascular smooth muscle. Implicated in cytokinesis, receptor capping, and cell locomotion..

Tissue specificity :Ubiquitously expressed in various hematopoietic cells..

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.