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HARS Rabbit mAb [UyR2]Cat NO.: A53170

Western blot(SDS PAGE) analysis of extracts from 293T cell lysate.Using HARS Rabbit mAb [UyR2]at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :HARS; HisRS; histidine translase; HRS; USH3B;

UniProtID :P12081

MASS(da) :57,411

MW(kDa) :50kDa

Form :Liquid

Purification :Affinity-chromatography

Host :Rabbit

Isotype : IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human HARS

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :293T cell lysate.

Function : Catalyzes the ATP-dependent ligation of histidine to the 3'-end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (His-AMP) (PubMed:29235198). Plays a role in axon guidance (PubMed:26072516)..

Tissue specificity :Brain, heart, liver and kidney.

Subcellular locationi :Cytoplasm.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.