IL1 beta Rabbit mAb [3g5n]Cat NO.: A28764

Protein names ：Catabolin; IFN beta inducing factor; IL 1 beta; IL1;IL1B; IL1F2; Interleukin 1 beta; Interleukin 1 beta precursor; interleukin 1, beta; Osteoclast activating factor; Preinterleukin 1 beta; Pro interleukin 1 beta;

UniProtID ：P10749（Mouse，抗原）

MASS(da) ：30,931

MW(kDa) ：31,28,17kDa

Form ：Liquid

Purification ：Affinity-chromatography

Host ：Rabbit

Isotype ： IgG

sensitivity ：Endogenous

Reactivity ：Mouse

• ApplicationDilution
• 免疫印迹(WB)1:1000-2000
• The optimal dilutions should be determined by the end user

Specificity ：Antibody is produced by immunizing animals with A synthesized peptide derived from human IL1 beta

Storage ：Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected ：RAW 264.7 cell treated with Lipopolysaccharide (LPS) cell lysate.

Function ： Potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore..

Tissue specificity ：Expressed in activated macrophages (at protein level)..

Subcellular locationi ：Cytoplasm, cytosol. Secreted. Lysosome. Secreted, extracellular exosome.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.