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Western blot(SDS PAGE) analysis of extracts from MCF-7 cells serum-starved overnight and then treated with IGF-1 (50 ng/ml).Using P-YB1 (S102) Rabbit mAb [2FKN] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :YBX1,NSEP1,YB1,YBOX1_HUMAN,Y-box-binding protein 1
UniProtID :P67809
MASS(da) :35,924
MW(kDa) :49 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human Phospho-YB1 (Ser102)
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :MCF-7 cells serum-starved overnight and then treated with IGF-1 (50 ng/ml)
Subcellular locationi :Cytoplasm. Nucleus. Cytoplasmic granule. Secreted. Secreted, extracellular exosome.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.