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Western blot(SDS PAGE) analysis of extracts from HepG2 cell lysate.Using R Cadherin Rabbit mAb [HuJA]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :Cadherin 4; cdh4; R CAD; Retinal cadherin;
UniProtID :P55283
MASS(da) :100,281
MW(kDa) :130kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human R Cadherin
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HepG2 cell lysate.
Function : Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells,cadherins may thus contribute to the sorting of heterogeneous cell types. May play an important role in retinal development.
Tissue specificity :Expressed mainly in brain but also found in other tissues.
Subcellular locationi :Cell membrane,Single-pass type I membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.