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BCKDK Rabbit mAb [0n3i]Cat NO.: A77069

Western blot(SDS PAGE) analysis of extracts from HeLa cell lysate.Using BCKDK Rabbit mAb [0n3i]at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :BCKD kinase; BCKDHKIN; Bckdk; BCKDKD; BDK;

UniProtID :O14874

MASS(da) :46,360

MW(kDa) :46kDa

Form :Liquid

Purification :Affinity-chromatography

Host :Rabbit

Isotype : IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human BCKDK

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HeLa cell lysate.

Function : Catalyzes the phosphorylation and inactivation of the branched-chain alpha-ketoacid dehydrogenase complex, the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. Key enzyme that regulate the activity state of the BCKD complex..

Tissue specificity :Ubiquitous.

Subcellular locationi :Mitochondrion matrix. Mitochondrion.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.