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Western blot(SDS PAGE) analysis of extracts from SH-SY5Y cell lysate.Using Glyt2 Rabbit mAb [1v8J]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :Glycine transporter; GlyT2; NET1; SC6AC5; Slc6a5; Slc6a9;
UniProtID :Q9Y345
MASS(da) :87,434
MW(kDa) :87kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Glyt2
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :SH-SY5Y cell lysate.
Function : Sodium- and chloride-dependent glycine transporter (PubMed:9845349, PubMed:10381548, PubMed:10606742, PubMed:31370103). Terminates the action of glycine by its high affinity sodium-dependent reuptake into presynaptic terminals (PubMed:9845349). May be responsible for the termination of neurotransmission at strychnine-sensitive glycinergic synapses (PubMed:9845349)..
Tissue specificity :Expressed in medulla, and to a lesser extent in spinal cord and cerebellum.
Subcellular locationi :Cell membrane,Multi-pass membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.