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Western blot(SDS PAGE) analysis of extracts from HepG2 cell lysate.Using IFT88 Rabbit mAb [F2j6]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :DAF19; hTg737; Ift88; TG737; TTC10;
UniProtID :Q13099
MASS(da) :93,192
MW(kDa) :94kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human IFT88
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HepG2 cell lysate.
Function : Involved in primary cilium biogenesis. Also involved in autophagy since it is required for trafficking of ATG16L and the expansion of the autophagic compartment..
Tissue specificity :Expressed in the heart, brain, liver, lung, kidney, skeletal muscle and pancreas..
Subcellular locationi :Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Cell projection, cilium. Cytoplasm, cytoskeleton, cilium basal body. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm. Cell projection, cilium, flagellum.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.