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Western blot(SDS PAGE) analysis of extracts from A431 cell lysate.Using NSDHL Rabbit mAb [D3iC]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :H105E3; NSDHL; SDR31E1; XAP104;
UniProtID :Q15738
MASS(da) :41,900
MW(kDa) :38kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human NSDHL
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :A431 cell lysate.
Function : Catalyzes the NAD(P)(+)-dependent oxidative decarboxylation of the C4 methyl groups of 4-alpha-carboxysterols in post-squalene cholesterol biosynthesis (By similarity). Plays also a role in the regulation of the endocytic trafficking of EGFR (By similarity)..
Tissue specificity :Brain, heart, liver, lung, kidney, skin and placenta.
Subcellular locationi :Endoplasmic reticulum membrane,Single-pass membrane protein. Lipid droplet.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.