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Western blot(SDS PAGE) analysis of extracts from THP-1 cell lysate.Using CFD Rabbit mAb [Vn76]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :Adipsin; And; CFD; Complement factor D; FactorD; PFD;
UniProtID :P00746
MASS(da) :27,033
MW(kDa) :25kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human CFD
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :THP-1 cell lysate.
Function : Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway.
Subcellular locationi :Secreted.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.