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Western blot(SDS PAGE) analysis of extracts from HepG2 cell lysate.Using PRD Rabbit mAb [ah04]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :Pep4; pepD; Peptidase 4; Peptidase D; Prolidase; Proline dipeptidase; X pro dipeptidase;
UniProtID :P12955
MASS(da) :54,548
MW(kDa) :55kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human PRD
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HepG2 cell lysate.
Function : Splits dipeptides with a prolyl or hydroxyprolyl residue in the C-terminal position. Plays an important role in collagen metabolism because the high level of iminoacids in collagen.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.