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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using HGF β Rabbit mAb [0F31] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :HGF,HPTA,HGF_HUMAN,Hepatocyte growth factor [Cleaved into: Hepatocyte growth factor alpha chain,Hepatocyte growth factor beta chain]
UniProtID :P14210
MASS(da) :83,134
MW(kDa) :35, 85 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human HGF β
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization..
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.