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Western blot(SDS PAGE) analysis of extracts from (1) Jurkat cell lysate; (2) Raw264.7 cell lysate.Using Annexin A7 Rabbit mAb [TW23]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :Annexin A7; Annexin VII; ANX7; ANXA7; SNX; Synexin;
UniProtID :P20073
MASS(da) :52,739
MW(kDa) :50kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Annexin A7
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :(1) Jurkat cell lysate; (2) Raw264.7 cell lysate.
Function : Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis.
Tissue specificity :Isoform 1 is expressed in brain, heart and skeletal muscle. Isoform 2 is more abundant in liver, lung, kidney, spleen, fibroblasts and placenta..
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.