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Western blot(SDS PAGE) analysis of extracts from (1) HepG2 cell lysate; (2) Mouse spleen lysate.Using ATE1 Rabbit mAb [S0e5]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :Arginyltransferase 1; Ate1; R transferase 1;
UniProtID :O95260
MASS(da) :59,090
MW(kDa) :59kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human ATE1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :(1) HepG2 cell lysate; (2) Mouse spleen lysate.
Function : Involved in the post-translational conjugation of arginine to the N-terminal aspartate or glutamate of a protein. This arginylation is required for degradation of the protein via the ubiquitin pathway. Does not arginylate cysteine residues (By similarity)..
Subcellular locationi :[Isoform ATE1-1]: Nucleus. Cytoplasm.,[Isoform ATE1-2]: Cytoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.