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Western blot(SDS PAGE) analysis of extracts from (1) HeLa cell lysate; (2) RAW 264.7 cell lysate.Using PMF1 Rabbit mAb [QYT7]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :Pmf1; Polyamine modulated factor 1;
UniProtID :Q6P1K2
MASS(da) :23,339
MW(kDa) :25kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human PMF1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :(1) HeLa cell lysate; (2) RAW 264.7 cell lysate.
Function : Part of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis. May act as a cotranscription partner of NFE2L2 involved in regulation of polyamine-induced transcription of SSAT..
Tissue specificity :Highest levels of expression in heart and skeletal muscle, with significant levels expressed in kidney and liver..
Subcellular locationi :Nucleus. Chromosome, centromere, kinetochore.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.