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Western blot(SDS PAGE) analysis of extracts from (1) A431 cell lysate; (2) NIH/3T3 cell lysate.Using SLC25A12 Rabbit mAb [9nYO]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :AGC1; ARALAR; ARALAR1; SLC25A12;
UniProtID :O75746
MASS(da) :74,762
MW(kDa) :72kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human SLC25A12
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :(1) A431 cell lysate; (2) NIH/3T3 cell lysate.
Function : Mitochondrial and calcium-binding carrier that catalyzes the calcium-dependent exchange of cytoplasmic glutamate with mitochondrial aspartate across the mitochondrial inner membrane (PubMed:11566871, PubMed:25410934). May have a function in the urea cycle (PubMed:11566871)..
Tissue specificity :Expressed predominantly in the heart and skeletal muscle, weakly in brain and kidney..
Subcellular locationi :Mitochondrion inner membrane,Multi-pass membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.