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Western blot(SDS PAGE) analysis of extracts from MCF7 cell lysate.Using Gamma glutamyl hydrolase Rabbit mAb [OIts]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :folylpolygammaglutamyl hydrolase; gamma glutamyl hydrolase;GGH;
UniProtID :Q92820
MASS(da) :35,964
MW(kDa) :36kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Gamma glutamyl hydrolase
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :MCF7 cell lysate.
Function : Hydrolyzes the polyglutamate sidechains of pteroylpolyglutamates. Progressively removes gamma-glutamyl residues from pteroylpoly-gamma-glutamate to yield pteroyl-alpha-glutamate (folic acid) and free glutamate (PubMed:11005824, PubMed:8816764). May play an important role in the bioavailability of dietary pteroylpolyglutamates and in the metabolism of pteroylpolyglutamates and antifolates..
Subcellular locationi :Secreted, extracellular space. Lysosome. Melanosome.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.