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Western blot(SDS PAGE) analysis of extracts from human serum cell lysate.Using Fetuin B Rabbit mAb [n1n7]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :Fet; Fetub; Fetuin beta; Gugu; Pp63;
UniProtID :Q9UGM5
MASS(da) :42,055
MW(kDa) :55kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Fetuin B
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :human serum cell lysate.
Function : Protease inhibitor required for egg fertilization. Required to prevent premature zona pellucida hardening before fertilization, probably by inhibiting the protease activity of ASTL, a protease that mediates the cleavage of ZP2 and triggers zona pellucida hardening (By similarity)..
Tissue specificity :Liver and testis.
Subcellular locationi :Secreted.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.