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Western blot(SDS PAGE) analysis of extracts from various lysates.Using P-PLCB3 (S537) Rabbit mAb [DN4X]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :Plcb3;
UniProtID :Q01970
MASS(da) :138,799
MW(kDa) :150kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Phospho-PLCB3 (S537)
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :various lysates
Function : The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes..
Subcellular locationi :Cytoplasm. Membrane. Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.