HRP-Glypican 3 Mouse mAb [HG13]Cat NO.: A12941

Protein names ：DGSX Glypican 3 glypican proteoglycan 3 glypican-3 GPC3 GTR2-2 heparan sulphate proteoglycan Intestinal protein OCI-5 MXR7 OCI5 OCI-5 secreted glypican-3 SGB SGBS SGBS1SDYS

UniProtID ：P51654

Form ：Liquid

Purification ：Protein A purified

Host ：Mouse

Isotype ：IgG1 Kappa

sensitivity ：Endogenous

Reactivity ：Human

• ApplicationDilution
• 免疫组化(IHC)1:100
• 免疫荧光(ICC/IF) 1:100
• The optimal dilutions should be determined by the end user

Specificity ：Recombinant fragment containing amino acids 511-580 of human Glypican 3

Storage ：Antibody store in 10 mM PBS . Shipped at 4°C. Store at 4C in the dark.. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

Function ：The Glypican 3 (GPC3) gene encodes for a member of the glypican-related integral membrane proteoglycan family (GRIPS). Two isoforms of the protein exist, isoform 1 at a length of 580 amino acids long and 65 kDA while isoform 2 is 526 amino acids long at 59 kDA. The protein encoded by GPC3 is thought to prevent the dipeptidyl peptidase activity of DPP4 and may participate in the suppression and regulation of growth in the predominately mesodermal tissues and organs. The GPC3 gene participates in the Wnt signaling pathway, metabolism, and visual phototransduction through interactions with genes FGF2, WNT3A, WNT7B, BCAN, and BGN. GPC3 has been researched in various diseases such as paine syndrome, horner's sundrome, liver cirrhosis, simpson-golabi-behmel syndrome, germ cell tumors, wilms tumors, complex regional pain syndrome, and hepatocellulcar carcinoma.

Tissue specificity ：Glypican-3 (GPC3) is an integral membrane protein that is mutated in the Simpson-Golabi-Behmel syndrome (SGBS). SGBS is characterized by pre- and post-natal overgrowth and is a recessive X-linked condition.GPC3 may also be found in a secreted form. Anti-GPC3 has been identified as a useful tumor marker for the diagnosis of hepatocellular carcinoma (HCC), hepatoblastoma, melanoma, testicular germ cell tumors, and Wilms tumor and hepatoblastoma, with a low or undetectable expression in normal adjacent tissue. In patients with thyroid cancer, expression of GPC3 is dramatically enhanced in certain types of cancers: 100% in follicular carcinoma and 70% in papillary carcinoma. Expression of GPC3 in follicular carcinoma was significantly higher than that of follicular adenoma. In contrast, GPC 3 is not expressed in anaplastic carcinoma.

Subcellular locationi ：Cytoplasmic

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.