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SDHA Rabbit mAb [L975]Cat NO.: A28087

Western blot(SDS PAGE) analysis of extracts from Wild-type HEK-293 cells .Using SDHARabbit mAb [L975] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :SDHA,SDH2,SDHF,SDHA_HUMAN,Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial

UniProtID :P31040

MASS(da) :72,692

MW(kDa) :72 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human SDHA

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :Wild-type HEK-293 cells 

Function : Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q) (PubMed:24781757). Can act as a tumor suppressor (PubMed:20484225)..

Subcellular locationi :Mitochondrion inner membrane,Peripheral membrane protein,Matrix side.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.