ATF3 Rabbit mAb [6AE2]Cat NO.: A58979

Protein names ：ATF3,ATF3_HUMAN,Cyclic AMP-dependent transcription factor ATF-3

UniProtID ：P18847

MASS(da) ：20,576

MW(kDa) ：21 kDa

Form ：Liquid

Purification ：Protein A purification

Host ：Rabbit

Isotype ：IgG

sensitivity ：Endogenous

Reactivity ：Human,Mouse

• ApplicationDilution
• 免疫印迹(WB)1:1000-2000
• 免疫组化(IHC)1:100
• 免疫荧光(ICC/IF) 1:100
• The optimal dilutions should be determined by the end user

Specificity ：Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human ATF3

Storage ：Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected ：THP-1 cells treated with 80nM TPA overnight then treated with 1µg/ml LPS for 8 h

Function ： This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter.., [Isoform 2]: Activates transcription presumably by sequestering inhibitory cofactors away from the promoters.., [Isoform 3]: Stress-induced isoform, counteracts the transcriptional repression of isoform 1..

Subcellular locationi ：Nucleus.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.