Occludin Rabbit mAb [7ST3]Cat NO.: A71200

Protein names ：OCLN,OCLN_HUMAN,Occludin

UniProtID ：Q16625

MASS(da) ：59,144

MW(kDa) ：23,53,65 kDa

Form ：Liquid

Purification ：Protein A purification

Host ：Rabbit

Isotype ：IgG

sensitivity ：Endogenous

Reactivity ：Human,Mouse,Rat

• ApplicationDilution
• 免疫印迹(WB)1:1000-2000
• 免疫组化(IHC)1:100
• 免疫荧光(ICC/IF) 1:100
• The optimal dilutions should be determined by the end user

Specificity ：Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Occludin

Storage ：Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected ：MDCK

Function ： May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions.., (Microbial infection) Acts as a coreceptor for hepatitis C virus (HCV) in hepatocytes..

Tissue specificity ：Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis..

Subcellular locationi ：Cell membrane,Multi-pass membrane protein. Cell junction, tight junction.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.