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Cyclin B1 Rabbit mAb [30J6]Cat NO.: A75224

Western blot(SDS PAGE) analysis of extracts from HeLa cells .Using Cyclin B1Rabbit mAb [30J6] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :CCNB1,CCNB,CCNB1_HUMAN,G2/mitotic-specific cyclin-B1

UniProtID :P14635

MASS(da) :48,337

MW(kDa) :58 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Cyclin B1

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HeLa cells 

Function : Essential for the control of the cell cycle at the G2/M (mitosis) transition..

Subcellular locationi :Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.