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Cytokeratin 7 Rabbit mAb [7FG0]Cat NO.: A99257

Western blot(SDS PAGE) analysis of extracts from SK-OV-3 cells .Using Cytokeratin 7Rabbit mAb [7FG0] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :KRT7,SCL,K2C7_HUMAN,Keratin, type II cytoskeletal 7

UniProtID :P08729

MASS(da) :51,386

MW(kDa) :51 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Cytokeratin 7

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :SK-OV-3 cells 

Function : Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7)..

Tissue specificity :Expressed in cultured epidermal, bronchial and mesothelial cells but absent in colon, ectocervix and liver. Observed throughout the glandular cells in the junction between stomach and esophagus but is absent in the esophagus..

Subcellular locationi :Cytoplasm.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.