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Proteasome 20S LMP2 Rabbit mAb [0UQW]Cat NO.: A53832

Western blot(SDS PAGE) analysis of extracts from  Wild-type HAP1 cells.Using Proteasome 20S LMP2Rabbit mAb [0UQW] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :PSMB9,LMP2,PSMB6i,RING12,PSB9_HUMAN,Proteasome subunit beta type-9

UniProtID :P28065

MASS(da) :23,264

MW(kDa) :21,23 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Proteasome 20S LMP2

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected : Wild-type HAP1 cells

Function : The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB6 by PSMB9 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues..

Subcellular locationi :Cytoplasm. Nucleus.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.