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FHL1 Rabbit mAb [04RZ]Cat NO.: A53618

Western blot(SDS PAGE) analysis of extracts from HEK293T cells .Using FHL1Rabbit mAb [04RZ] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :FHL1,SLIM1,FHL1_HUMAN,Four and a half LIM domains protein 1

UniProtID :Q13642

MASS(da) :36,263

MW(kDa) :32 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human FHL1

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HEK293T cells 

Function : May have an involvement in muscle development or hypertrophy.

Tissue specificity :Isoform 1 is highly expressed in skeletal muscle and to a lesser extent in heart, placenta, ovary, prostate, testis, small intestine, colon and spleen. Expression is barely detectable in brain, lung, liver, kidney, pancreas, thymus and peripheral blood leukocytes. Isoform 2 is expressed in brain, skeletal muscle and to a lesser extent in heart, colon, prostate and small intestine. Isoform 3 is expressed in testis, heart and skeletal muscle..

Subcellular locationi :[Isoform 1]: Cytoplasm.,[Isoform 3]: Cytoplasm. Nucleus.,[Isoform 2]: Nucleus. Cytoplasm, cytosol.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.