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Tissue Factor Rabbit mAb [0NEB]Cat NO.: A98687

Western blot(SDS PAGE) analysis of extracts from Wild-type HAP1 cells .Using Tissue FactorRabbit mAb [0NEB] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :F3,TF_HUMAN,Tissue factor

UniProtID :P13726

MASS(da) :33,068

MW(kDa) :47 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Tissue Factor

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :Wild-type HAP1 cells 

Function : Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited proteolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade..

Tissue specificity :Lung, placenta and pancreas..

Subcellular locationi :[Isoform 1]: Membrane,Single-pass type I membrane protein.,[Isoform 2]: Secreted.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.