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Cyclin E2 Rabbit mAb [Q252]Cat NO.: A60711

Western blot(SDS PAGE) analysis of extracts from MCF7 CELLS.Using Cyclin E2Rabbit mAb [Q252] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :CCNE2,CCNE2_HUMAN,G1/S-specific cyclin-E2

UniProtID :O96020

MASS(da) :46,757

MW(kDa) :45 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human Cyclin E2

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :MCF7 CELLS

Function : Essential for the control of the cell cycle at the late G1 and early S phase.

Tissue specificity :According to PubMed:9858585, highest levels of expression in adult testis, thymus and brain. Lower levels in placenta, spleen and colon. Consistently elevated levels in tumor-derived cells compared to non-transformed proliferating cells. According to PubMed:9840927: low levels in thymus, prostate, brain, skeletal muscle, and kidney. Elevated levels in lung. According to PubMed:9840943 highly expressed in testis, placenta, thymus and brain. In a lesser extent in small intestine and colon.

Subcellular locationi :Nucleus.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.