IL-1 beta Rabbit mAb [B789]Cat NO.: A13948

Protein names ：IL1B,IL1F2,IL1B_HUMAN,Interleukin-1 beta

UniProtID ：P01584

MASS(da) ：30,748

MW(kDa) ：31/28/17.5 kDa

Form ：Liquid

Purification ：Protein A purification

Host ：Rabbit

Isotype ：IgG

sensitivity ：Endogenous

Reactivity ：Human,Mouse,Rat

• ApplicationDilution
• 免疫印迹(WB)1:1000-2000
• The optimal dilutions should be determined by the end user

Specificity ：Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human IL-1 beta

Storage ：Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected ：Raw 264.7 cells treated with 100 ng/ml LPS for 7 h and 300 ng/ml Brefeldin A for the last 3h

Function ： Potent pro-inflammatory cytokine (PubMed:3920526, PubMed:10653850, PubMed:12794819). Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production (PubMed:3920526). Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells (PubMed:10653850). Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6 (PubMed:12794819). Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore (PubMed:33377178, PubMed:33883744)..

Tissue specificity ：Expressed in activated monocytes/macrophages (at protein level)..

Subcellular locationi ：Cytoplasm, cytosol. Secreted. Lysosome. Secreted, extracellular exosome.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.