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CPT2 Rabbit mAb [40G2]Cat NO.: A96396

Western blot(SDS PAGE) analysis of extracts from MCF7 CELLS .Using CPT2Rabbit mAb [40G2] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :CPT2,CPT1,CPT2_HUMAN,Carnitine O-palmitoyltransferase 2, mitochondrial

UniProtID :P23786

MASS(da) :73,777

MW(kDa) :67 kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human CPT2

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :MCF7 CELLS 

Function : Involved in the intramitochondrial synthesis of acylcarnitines from accumulated acyl-CoA metabolites (PubMed:20538056, PubMed:24780397). Reconverts acylcarnitines back into the respective acyl-CoA esters that can then undergo beta-oxidation, an essential step for the mitochondrial uptake of long-chain fatty acids and their subsequent beta-oxidation in the mitochondrion. Active with medium (C8-C12) and long-chain (C14-C18) acyl-CoA esters (PubMed:20538056)..

Subcellular locationi :Mitochondrion inner membrane,Peripheral membrane protein,Matrix side.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.