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Western blot(SDS PAGE) analysis of extracts from HEK293T cells.Using HNRPABRabbit mAb [8KG3] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :HNRNPAB,ABBP1,HNRPAB,ROAA_HUMAN,Heterogeneous nuclear ribonucleoprotein A/B
UniProtID :Q99729
MASS(da) :36,225
MW(kDa) :42 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of human HNRPAB
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HEK293T cells
Function : Binds single-stranded RNA. Has a high affinity for G-rich and U-rich regions of hnRNA. Also binds to APOB mRNA transcripts around the RNA editing site.
Tissue specificity :Ubiquitous.
Subcellular locationi :Nucleus. Cytoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.